Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq.

Philpott M., Watson J., Thakurta A., Brown T., Brown T., Oppermann U., Cribbs AP.

Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells. Barcodes and unique molecular identifiers are synthesized using dimeric nucleotide building blocks that allow error detection. We illustrate the use of the method for evaluating barcode assignment accuracy, differential isoform usage in myeloma cell lines, and fusion transcript detection in a sarcoma cell line.

DOI

10.1038/s41587-021-00965-w

Type

Journal article

Journal

Nat biotechnol

Publication Date

12/2021

Volume

39

Pages

1517 - 1520

Keywords

High-Throughput Nucleotide Sequencing, Nanopore Sequencing, Protein Isoforms, Transcriptome

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