Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Neutrophils display distinct gene expression patters depending on their developmental stage, activation state and tissue microenvironment. To determine the transcription factor networks that shape these responses in a mouse model, we integrated transcriptional and chromatin analyses of neutrophils during acute inflammation. We showed active chromatin remodeling at two transition stages: bone marrow-to-blood and blood-to-tissue. Analysis of differentially accessible regions revealed distinct sets of putative transcription factors associated with control of neutrophil inflammatory responses. Using ex vivo and in vivo approaches, we confirmed that RUNX1 and KLF6 modulate neutrophil maturation, whereas RELB, IRF5 and JUNB drive neutrophil effector responses and RFX2 and RELB promote survival. Interfering with neutrophil activation by targeting one of these factors, JUNB, reduced pathological inflammation in a mouse model of myocardial infarction. Therefore, our study represents a blueprint for transcriptional control of neutrophil responses in acute inflammation and opens possibilities for stage-specific therapeutic modulation of neutrophil function in disease.

Original publication

DOI

10.1038/s41590-021-00968-4

Type

Journal article

Journal

Nat immunol

Publication Date

09/2021

Volume

22

Pages

1093 - 1106

Keywords

Animals, CHO Cells, Cell Line, Chromatin Assembly and Disassembly, Core Binding Factor Alpha 2 Subunit, Cricetulus, Female, Inflammation, Interferon Regulatory Factors, Kruppel-Like Factor 6, Mice, Mice, Inbred C57BL, Myocardial Infarction, Neutrophils, Regulatory Factor X Transcription Factors, Transcription Factor RelB, Transcription Factors, Transcription, Genetic, Transcriptional Activation