Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

A commonly used monoclonal antibody targeting osteoprotegerin (OPG), MAB8051, detects a truncated protein species in breast and prostate cancer cell lysates. OPG expression has been reported to contribute to cell survival of both of these cancers. We hypothesised that the truncated protein represented a unique tumour-associated OPG isoform. However, here we show that the truncated protein identified by MAB8051 in cancer cell lines is carbonic anhydrase II (CA II), also implicated in tumour biology. We clearly demonstrate cross-reactivity of this OPG antibody in western blots. OPG and CA II RNA-interference studies confirmed the identity of the bands. We show almost identical staining patterns between MAB8051 and CA II immunohistochemistry of different human tissue types and human tumour types using serial sections. We conclude that care should be exercised using this antibody for immunohistochemistry studies, without additional in situ hybridisation, or parallel use of other OPG-specific antibodies.

Original publication

DOI

10.1002/ijc.22946

Type

Journal article

Journal

Int j cancer

Publication Date

01/11/2007

Volume

121

Pages

1958 - 1966

Keywords

Amino Acid Sequence, Antibodies, Monoclonal, Carbonic Anhydrase II, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, Molecular Sequence Data, Molecular Weight, Neoplasms, Osteoprotegerin, Protein Processing, Post-Translational, RNA, Messenger, RNA, Small Interfering