Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Lipopolysaccharide (LPS) signals through Toll-like receptors (TLRs) in the course of sepsis, resulting in the release of inflammatory factors. In cell lines and murine models, parts of the signaling pathways involved have been elucidated with MyD88, Mal/TIRAP and IKK2 playing an important role in the induction of NF-kappaB. By focusing on primary human cells, we have shown that there are fundamental signaling differences between human and murine macrophages and between cells of myeloid and non-myeloid origins. In primary human cells, there are no available knockouts so we employed the use of dominant negatives to investigate the signaling cascades. We show that in primary human macrophages MyD88, Mal/TIRAP and IKK2-independent alternative pathways activate NF-kappaB and induce the expression of inflammatory cytokines, whereas in non-myeloid synovial fibroblasts MyD88 and/or Mal/TIRAP are essential adaptors for LPS signaling.

Original publication

DOI

10.1179/096805104225005878

Type

Journal article

Journal

J endotoxin res

Publication Date

2004

Volume

10

Pages

445 - 452

Keywords

Adaptor Proteins, Signal Transducing, Adenoviridae, Animals, Antigens, Differentiation, Cells, Cultured, Dose-Response Relationship, Drug, Fibroblasts, Flow Cytometry, Humans, I-kappa B Kinase, Lipopolysaccharides, Macrophages, Membrane Glycoproteins, Mice, Myeloid Differentiation Factor 88, NF-kappa B, Protein-Serine-Threonine Kinases, Receptors, Cell Surface, Receptors, Immunologic, Receptors, Interleukin-1, Signal Transduction, Species Specificity, Synovial Membrane, Toll-Like Receptors