Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We report a sensitivity of the osteoclast cell surface Ca2+ receptor to extracellular protons. Freshly isolated rat osteoclasts were exposed to the known agonists of the Ca2+ receptor, Ca2+ and Ni2+, in extracellular solutions set at different pH values. Decreasing the extracellular pH from 7.8 to 4.0 units markedly potentiated the cytosolic Ca2+ signals elicited in response to Ca2+ receptor activation by either Ni2+ (50 microM, 500 microM or 5 mM) or Ca2+ (5 mM). Each response consisted of a rapid and usually transient elevation of cytosolic [Ca2+]. Maximal cytosolic [Ca2+] responses were obtained at pH values of 6.6 (for 5 mM-[Ni2+]) and 4.0 units (for 5 mM-[Ca2+]). Finally, the effects of extracellular pH persisted in Ca(2+)-free, EGTA-containing solutions, suggesting a modulation of intracellular Ca2+ release.

Original publication

DOI

10.1006/bbrc.1994.1291

Type

Journal article

Journal

Biochemical and biophysical research communications

Publication Date

03/1994

Volume

199

Pages

742 - 747

Addresses

Bone Unit, St. George's Hospital Medical School, London, U.K.

Keywords

Cells, Cultured, Cell Membrane, Cytosol, Osteoclasts, Animals, Animals, Newborn, Rats, Rats, Wistar, Calcium, Nickel, Egtazic Acid, Fura-2, Calcium-Binding Proteins, Spectrometry, Fluorescence, Hydrogen-Ion Concentration