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We report that the divalent cation Ni2+ elicits elevations in the cytosolic free Ca2+ concentration ([Ca2+]) in cultured enterocytes. These elevations were monophasic, each response consisting of a rapid initial transient rise of cytosolic [Ca2+] to a peak value followed by an exponential decline. The magnitude of the cytosolic [Ca2+] elevation varied with the concentration of applied Ni2+. In some cells, a single application of Ni2+ induced oscillatory changes in cytosolic [Ca2+]. There was also evidence for use-dependent inactivation: a conditioning application of Ni2+ substantially attenuated the response resulting from its subsequent application. Our findings thus demonstrate the existence of a divalent cation-sensing "receptor" on the enterocyte. This putative receptor may play a role in regulating mineral absorption across the enterocyte membrane.

Original publication

DOI

10.1006/bbrc.1995.1748

Type

Journal article

Journal

Biochemical and biophysical research communications

Publication Date

05/1995

Volume

210

Pages

948 - 953

Addresses

Bone Research Unit, St. George's Hospital Medical School, London, UK.

Keywords

Colon, Epithelium, Cell Line, Cytosol, Humans, Cations, Divalent, Calcium, Nickel, Receptors, Cell Surface, Oscillometry, Kinetics, Time Factors