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We demonstrated previously that osteoclasts possess a divalent cation-sensitive "receptor", the Ca2+ receptor. Activation of the Ca2+ receptor by the surrogate cation Ni2+ was shown to elicit an increase in cytosolic [Ca2+] to a peak value followed by an exponential decline. In the present study we examined the influence of surface membrane voltage on the kinetics of Ca2+ receptor inactivation. The K+ ionophore, valinomycin was applied to intercept the declining phase of the cytosolic [Ca2+] transient elicited by application of between 50 microM- and 5 mM-[Ni2+]. This resulted in a sustained elevation of cytosolic [Ca2+] or even a 'hump' followed by a gradual decline. Such a kinetic alteration persisted in a Ca(2+)-free solution, but was abolished in high extracellular [K+] (105 mM). Thus, we demonstrate for the first time to our knowledge, a modulatory effect of membrane potential on the function of the osteoclast Ca2+ receptor.

Original publication




Journal article


Biochem biophys res commun

Publication Date





1100 - 1105


Animals, Animals, Newborn, Calcium, Calcium-Binding Proteins, Cell Membrane, Cells, Cultured, Cytosol, Fura-2, Kinetics, Membrane Potentials, Models, Biological, Osteoclasts, Potassium, Rats, Rats, Wistar, Time Factors, Valinomycin