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We demonstrated previously that osteoclasts possess a divalent cation-sensitive "receptor", the Ca2+ receptor. Activation of the Ca2+ receptor by the surrogate cation Ni2+ was shown to elicit an increase in cytosolic [Ca2+] to a peak value followed by an exponential decline. In the present study we examined the influence of surface membrane voltage on the kinetics of Ca2+ receptor inactivation. The K+ ionophore, valinomycin was applied to intercept the declining phase of the cytosolic [Ca2+] transient elicited by application of between 50 microM- and 5 mM-[Ni2+]. This resulted in a sustained elevation of cytosolic [Ca2+] or even a 'hump' followed by a gradual decline. Such a kinetic alteration persisted in a Ca(2+)-free solution, but was abolished in high extracellular [K+] (105 mM). Thus, we demonstrate for the first time to our knowledge, a modulatory effect of membrane potential on the function of the osteoclast Ca2+ receptor.

Original publication

DOI

10.1006/bbrc.1993.1530

Type

Journal article

Journal

Biochemical and biophysical research communications

Publication Date

05/1993

Volume

192

Pages

1100 - 1105

Addresses

Bone Research Unit, St. George's Hospital Medical School, London, U.K.

Keywords

Cells, Cultured, Cell Membrane, Cytosol, Osteoclasts, Animals, Animals, Newborn, Rats, Rats, Wistar, Potassium, Calcium, Fura-2, Valinomycin, Calcium-Binding Proteins, Membrane Potentials, Kinetics, Models, Biological, Time Factors