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We have investigated the effect of the alkaloid ryanodine on the release of intracellularly stored Ca2+ in response to activation of the osteoclast Ca2+ receptor by the surrogate agonist, Ni2+, Ni2+ (6 mM) in the presence of ethylene-glycol bis-(aminoethyl ether) tetraacetic acid (EGTA) (1.2 mM) and valinomycin (5 microM) induced a transient elevation of cytosolic [Ca2+] in fura 2-loaded osteoclasts. This transient was superimposed upon a small steady elevation of cytosolic [Ca2+] induced by the initial application of valinomycin alone. Ryanodine (10 microM) completely abolished such responsiveness. However, cytosolic [Ca2+] transients were restored when osteoclasts were depolarized by the extracellular inclusion of 100 mM-[K+] in the same solution. Thus, we demonstrate a sensitivity of the osteoclast signal transduction system to ryanodine for the first time to our knowledge.

Type

Journal article

Journal

Biochemical and biophysical research communications

Publication Date

11/1992

Volume

188

Pages

1332 - 1336

Addresses

Bone and Mineral Metabolism Unit, St. George's Hospital Medical School, London, U.K.

Keywords

Cells, Cultured, Cytosol, Osteoclasts, Animals, Rats, Inbred Strains, Animals, Newborn, Rats, Rats, Wistar, Calcium, Nickel, Valinomycin, Calcium-Binding Proteins, Ryanodine Receptor Calcium Release Channel, Receptors, Cholinergic, Signal Transduction