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5,6-dimethylxanthenone-4-acetic acid (DMXAA), currently in phase I trials, demonstrates excellent activity against transplantable murine tumours with established vasculature. The induction of cytokines, particularly of tumour necrosis factor (TNF), appears to be critical to its action. We investigated TNF induction by DMXAA in cultured human peripheral blood leucocytes (HPBL). TNF was measured by an enzyme-linked immunosorbent assay after 8 h, and NF-kappaB induction by electrophoretic mobility shift assays (EMSA) after 2 h. DMXAA (800 microg/ml) had no effect alone on TNF production but augmented, by up to 4-fold, the ability of bacterial lipopolysaccharide (LPS) to induce TNF. Previously reported results showing TNF production by DMXAA alone were traced to the presence in an earlier batch of DMXAA of a small amount of LPS, the action of which could be blocked by polymyxin B. DMXAA stimulated TNF production by deacylated LPS, which alone had little effect. An antibody (MEM-18) to the CD14 receptor, while blocking the induction of TNF by LPS, enabled DMXAA to both synthesise TNF and induce NF-kappaB. The structurally related drug, flavone acetic acid (FAA), did not induce TNF or synergise with anti-CD14 antibody. DMXAA strongly augmented the ability of suboptimal concentrations of interleukin-1 (IL-1) (25 ng/ml), okadaic acid (OA) (20 ng/ml) and phorbol-12-myristate-13-acetate (PMA) (5 ng/ml) to induce TNF production, suggesting that it affects multiple pathways converging on NF-kappaB activation. Sodium salicylate, a drug reported to inhibit the beta-subunit of IkappaB kinase (IKK), appeared to competitively inhibit TNF production by DMXAA in the presence of anti-CD14 antibody. Taken together, the results indicate DMXAA acts in vitro on HPBL to co-stimulate TNF production by a wide variety of agents, and suggests that IKK is the target that mediates this action.

Original publication

DOI

10.1016/s0959-8049(01)00210-6

Type

Journal article

Journal

European journal of cancer (Oxford, England : 1990)

Publication Date

10/2001

Volume

37

Pages

1930 - 1937

Addresses

Auckland Cancer Society Research Centre, University of Auckland School of Medicine, Auckland, New Zealand.

Keywords

Leukocytes, Mononuclear, Humans, Salicylic Acid, Okadaic Acid, Tetradecanoylphorbol Acetate, Xanthenes, Xanthones, Lipopolysaccharides, Tumor Necrosis Factor-alpha, NF-kappa B, Antigens, CD14, Antineoplastic Agents, Cytokines, Enzyme Inhibitors, Enzyme-Linked Immunosorbent Assay, Cell Culture Techniques, Dose-Response Relationship, Drug, Drug Synergism