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During infection, the release of damage-associated molecular patterns, so-called "alarmins," orchestrates the immune response. The alarmin IL-33 plays a role in a wide range of pathologies. Upon release, IL-33 signals through its receptor ST2, which reportedly is expressed only on CD4(+) T cells of the Th2 and regulatory subsets. Here we show that Th1 effector cells also express ST2 upon differentiation in vitro and in vivo during lymphocytic choriomeningitis virus (LCMV) infection. The expression of ST2 on Th1 cells was transient, in contrast to constitutive ST2 expression on Th2 cells, and marked highly activated effector cells. ST2 expression on virus-specific Th1 cells depended on the Th1-associated transcription factors T-bet and STAT4. ST2 deficiency resulted in a T-cell-intrinsic impairment of LCMV-specific Th1 effector responses in both mixed bone marrow-chimeric mice and adoptive cell transfer experiments. ST2-deficient virus-specific CD4(+) T cells showed impaired expansion, Th1 effector differentiation, and antiviral cytokine production. Consequently, these cells mediated little virus-induced immunopathology. Thus, IL-33 acts as a critical and direct cofactor to drive antiviral Th1 effector cell activation, with implications for vaccination strategies and immunotherapeutic approaches.

Type

Journal article

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Date

17/03/2015

Volume

112

Pages

4056 - 4061

Addresses

Experimental Immunology, Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany; German Rheumatism Research Center Berlin, 10117 Berlin, Germany;

Keywords

T-Lymphocytes, Th1 Cells, CD8-Positive T-Lymphocytes, Animals, Mice, Inbred C57BL, Mice, Lymphocytic choriomeningitis virus, Arenaviridae Infections, T-Box Domain Proteins, Antigens, CD44, L-Selectin, Interleukins, Flow Cytometry, Cell Separation, Gene Expression Profiling, Cell Differentiation, Gene Expression Regulation, STAT4 Transcription Factor, Interferon-gamma