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Fluorescence Recovery After Photobleaching (FRAP) is an established method for validating chemical probes against the chromatin reading bromodomains, but so far requires constant human supervision. Here, we present Frapid, an automated open source code implementation of FRAP that fully handles cell identification through fuzzy logic analysis, drug dispensing with a custom-built fluid handler, image acquisition & analysis, and reporting. We successfully tested Frapid on 3 bromodomains as well as on spindlin1 (SPIN1), a methyl lysine binder, for the first time.

Original publication




Journal article


Biomed opt express

Publication Date





422 - 441


(100.3008) Image recognition, algorithms and filters, (150.0150) Machine vision, (150.5758) Robotic and machine control, (170.1530) Cell analysis, (170.1790) Confocal microscopy, (170.2520) Fluorescence microscopy