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Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion incurred by ventilation and cardiac contractions. In this chapter, we report protocols for imaging ex vivo live lung slices and the intact mouse lung.

Original publication




Journal article


Curr protoc cytom

Publication Date



Chapter 12


Animals, Cell Movement, Diagnostic Imaging, Fluorescent Antibody Technique, Green Fluorescent Proteins, Instillation, Drug, Lung, Mice, Microscopy, Staining and Labeling