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K+ channels are widely expressed in eukaryotic and prokaryotic cells, where one of their key functions is to set the membrane potential. Many K+ channels are tetramers that share common architectural properties. The crystal structure of bacterial and mammalian K+ channels has been resolved and provides the basis for modeling their three-dimensional structure in different functional states. This wealth of information on K+ channel structure contrasts with the difficulties to visualize single K+ channel proteins in their physiological environment. We describe a method to identify single Ca2+-activated K+ channel molecules in the plasma membrane of migrating cells. Our method is based on dual-color labeling with quantum dots. We show that >90% of the observed quantum dots correspond to single K+ channel proteins. We anticipate that our method can be adopted to label any other ion channel in the plasma membrane on the single molecule level.

Original publication

DOI

10.1152/ajpcell.00633.2005

Type

Journal article

Journal

American Journal of Physiology - Cell Physiology

Publication Date

08/2006

Volume

291

Pages

C266 - C269

Addresses

Institute of Physiology II, Univ. of Münster, Robert-Koch-Strasse 27b, D-48149 Münster, Germany.

Keywords

Kidney, Cell Line, Cell Membrane, Animals, Dogs, Potassium Channels, Calcium-Activated, Microscopy, Fluorescence, Multiphoton, Microchemistry, Staining and Labeling, Quantum Dots