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Since the discovery that neural tissue contains a population of stem cells that form neurospheres in vitro, sphere-forming assays have been adapted for use with a number of different tissue types for the quantification of stem cell activity and self-renewal. One tissue type widely used for stem cell investigations is mammary tissue, and the mammosphere assay has been used in both normal tissue and cancer. Although it is a relatively simple assay to learn, it can be difficult to master. There are methodological and analytical aspects to the assay which require careful consideration when interpreting the results. We describe here a detailed mammosphere assay protocol for the assessment of stem cell activity and self-renewal, and discuss how data generated by the assay can be analysed and interpreted.

Original publication

DOI

10.1007/s10911-012-9255-3

Type

Journal article

Journal

Journal of mammary gland biology and neoplasia

Publication Date

05/06/2012

Volume

17

Pages

111 - 117

Addresses

Breast Biology Group, School of Cancer and Enabling Sciences, Paterson Institute for Cancer Research, The University of Manchester, Wilmslow Road, Manchester, UK.

Keywords

Mammary Glands, Human, Mammary Glands, Animal, Cell Line, Cell Line, Tumor, Animals, Humans, Carcinoma, Intraductal, Noninfiltrating, Breast Neoplasms, Tumor Stem Cell Assay, Cell Proliferation, Female, Neoplastic Stem Cells