Degeneration of the knee joint during osteoarthritis often begins with meniscal lesions. Meniscectomy, previously performed extensively after meniscal injury, is now obsolete because of the inevitable osteoarthritis that occurs following this procedure. Clinically, meniscus self-renewal is well documented as long as the outer, vascularized meniscal ring remains intact. In contrast, regeneration of the inner, avascular meniscus does not occur. Here, we show that cartilage tissue harvested from the avascular inner human meniscus during the late stages of osteoarthritis harbors a unique progenitor cell population. These meniscus progenitor cells (MPCs) are clonogenic and multipotent and exhibit migratory activity. We also determined that MPCs are likely to be controlled by canonical transforming growth factor β (TGF-β) signaling that leads to an increase in SOX9 and a decrease in RUNX2, thereby enhancing the chondrogenic potential of MPC. Therefore, our work is relevant for the development of novel cell biological, regenerative therapies for meniscus repair.
Stem cell reports
789 - 803
Aged, Cell Culture Techniques, Cell Differentiation, Cell Movement, Cells, Cultured, Chondrocytes, Core Binding Factor Alpha 1 Subunit, Gene Expression Profiling, Humans, Immunoblotting, Immunohistochemistry, Menisci, Tibial, Middle Aged, Oligonucleotide Array Sequence Analysis, Osteoarthritis, Proteome, Proteomics, Reverse Transcriptase Polymerase Chain Reaction, SOX9 Transcription Factor, Signal Transduction, Stem Cells, Tissue Engineering, Transforming Growth Factor beta3