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Near-infrared spectroscopy is a widely adopted technique for characterising biological tissues. The high dimensionality of spectral data, however, presents a major challenge for analysis. Here, we present a second-derivative Beer's law-based technique aimed at projecting spectral data onto a lower dimension feature space characterised by the constituents of the target tissue type. This is intended as a preprocessing step to provide a physically-based, low dimensionality input to predictive models. Testing the proposed technique on an experimental set of 145 bovine cartilage samples before and after enzymatic degradation, produced a clear visual separation between the normal and degraded groups. Reduced proteoglycan and collagen concentrations, and increased water concentrations were predicted by simple linear fitting following degradation (all [Formula: see text]). Classification accuracy using the Mahalanobis distance was [Formula: see text] between these groups.

Original publication




Journal article


Biomed phys eng express

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cartilage, near infrared spectroscopy, osteoarthritis