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Recently developed latest version of the sequence-directed single-base resolution nucleosome mapping reveals existence of strong nucleosomes and chromatin columnar structures (columns). Broad application of this simple technique for further studies of chromatin and chromosome structure requires some basic understanding as to how it works and what information it affords. The paper provides such an introduction to the method. The oscillating maps of singular nucleosomes, of short and long oligonucleosome columns, are explained, as well as maps of chromatin on satellite DNA and occurrences of counter-phase (antiparallel) nucleosome neighbors.

Original publication

DOI

10.1080/07391102.2014.958877

Type

Journal article

Journal

Journal of biomolecular structure & dynamics

Publication Date

01/2015

Volume

33

Pages

1558 - 1566

Addresses

a Institute of Evolution , University of Haifa , Haifa , Israel.

Keywords

Chromatin, Nucleosomes, Animals, Base Sequence