B1a B cells require autophagy for metabolic homeostasis and self-renewal.

Clarke AJ., Riffelmacher T., Braas D., Cornall RJ., Simon AK.

Specific metabolic programs are activated by immune cells to fulfill their functional roles, which include adaptations to their microenvironment. B1 B cells are tissue-resident, innate-like B cells. They have many distinct properties, such as the capacity to self-renew and the ability to rapidly respond to a limited repertoire of epitopes. The metabolic pathways that support these functions are unknown. We show that B1 B cells are bioenergetically more active than B2 B cells, with higher rates of glycolysis and oxidative phosphorylation, and depend on glycolysis. They acquire exogenous fatty acids and store lipids in droplet form. Autophagy is differentially activated in B1a B cells, and deletion of the autophagy gene Atg7 leads to a selective loss of B1a B cells caused by a failure of self-renewal. Autophagy-deficient B1a B cells down-regulate critical metabolic genes and accumulate dysfunctional mitochondria. B1 B cells, therefore, have evolved a distinct metabolism adapted to their residence and specific functional properties.

DOI

10.1084/jem.20170771

Type

Journal article

Journal

J exp med

Publication Date

05/02/2018

Volume

215

Pages

399 - 413

Keywords

Animals, Autophagy, Autophagy-Related Protein 7, B-Lymphocyte Subsets, Cell Lineage, Cell Self Renewal, Cell Survival, Fatty Acids, Female, Glycolysis, Homeostasis, Lipid Metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Oxidative Phosphorylation

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