Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

microRNAs regulate gene expression and influence the pathogenesis of human diseases. The present study investigated miR-21 in the pathogenesis of intestinal inflammation, as miR-21 is highly expressed in Inflammatory Bowel Disease (IBD). IBD is associated with intestinal barrier dysfunction and an altered gut microbiota. Recent studies have demonstrated that host microRNAs can shape the microbiota. Thus, we determined the influence of miR-21 on the gut microbiota and subsequent impact in a dextran sodium sulphate (DSS)-induced colitis model.The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type (WT) and miR-21-/- mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free (GF) mice with fecal homogenate, prior to DSS administration. 16S rRNA sequencing was performed on WT and miR-21-/- mice to dissect potential differences in the gut microbiota.miR-21-/- mice have reduced susceptibility to DSS-induced colitis compared to WT mice. Co-housing conferred some protection to WT mice, while GF mice colonized with fecal homogenate from miR-21-/- were protected from DSS colitis compared to those colonized with WT homogenate. Further supporting a role for the microbiota in the observed phenotype, the protection afforded by miR-21 depletion is lost when mice are pre-treated with antibiotics. 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21-/- intestinal microbiota.These findings suggest that miR-21 influences pathogenesis of intestinal inflammation by causing propagation of a disrupted gut microbiota.

Original publication




Journal article


Journal of Crohn's & colitis

Publication Date



School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Ireland.