In Vivo Imaging of MMP‐13 Activity Using a Specific Polymer‐FRET Peptide Conjugate Detects Early Osteoarthritis and Inhibitor Efficacy
Duro‐Castano A., Lim NH., Tranchant I., Amoura M., Beau F., Wieland H., Kingler O., Herrmann M., Nazaré M., Plettenburg O., Dive V., Vicent MJV., Nagase H.
Imaging early molecular changes in osteoarthritic (OA) joints is instrumental for the development of disease-modifying drugs. To this end, a ﬂuorescent resonance energy transfer-based peptide probe that is cleavable by matrix metalloproteinase 13 (MMP-13) has been developed. This protease degrades type II collagen, a major matrix component of cartilage. The probe exhibits high catalytic efﬁciency (kcat/KM = 6.5 × 105 M−1 s−1) and high selectivity for MMP-13 over a set of nine MMPs. To achieve optimal in vivo pharmacoki-netics and tissue penetration, the probe has been further conjugated to a linear L-polyglutamate chain of 30 kDa. The conjugate detects early bio-chemical events that occur in a surgically induced murine model of OA before major histological changes. The nanometric probe is suitable for the moni-toring of in vivo efﬁcacy of an orally bioavailable MMP-13 inhibitor, which effectively blocks cartilage degradation during the development of OA. This new polymer-probe can therefore be a useful tool in detecting early OA, disease progression, and in developing MMP-13-based disease-modifying drugs for OA.