Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.
Proc natl acad sci u s a
13204 - 13209
label-free, photonic biosensing, photonic crystal, single-cell analysis, Animals, Biosensing Techniques, Cells, Cultured, Cricetinae, Hep G2 Cells, Humans, Image Processing, Computer-Assisted, Kidney, Photons, Signal Transduction, Single-Cell Analysis, Thrombopoietin