Primary cilia regulate and coordinate a variety of cell signaling pathways important in chondrocyte physiology and cartilage development, health, and disease. Despite this, the chondrocyte primary cilium and its associated role in cartilage biology remains poorly understood. Key to elucidating primary cilia structure and function in chondrocytes is the ability to visualize this unique structure. Here we describe materials and methods for immunofluorescence labeling, microscopy, and measurement of chondrocyte primary cilia.
Journal article
2023-01-01T00:00:00+00:00
2598
157 - 176
19
Chondrocyte, Confocal microscopy, Immunofluorescence, Primary cilia, Quantification, Super resolution, Chondrocytes, Cartilage, Articular, Cilia, Signal Transduction