Single-cell sequencing allows for the measurement of sequence information from individual cells with next-generation sequencing (NGS). However, its application to third-generation sequencing platforms such as Oxford Nanopore has been challenging because of its lower basecalling accuracy. Here we describe the method to perform highly accurate single-cell COrrected Long-Read sequencing (scCOLOR-seq) by droplet-based encapsulation of cells and sequencing using the Oxford Nanopore Sequencing system.
Journal article
2023-01-01T00:00:00+00:00
2632
259 - 267
8
Long-read, Oxford Nanopore Sequencing, Single-cell, High-Throughput Nucleotide Sequencing, Sequence Analysis, DNA, Nanopores