Synthetic substrates with defined chemical and structural characteristics may potentially be prepared to mimic the living ECM to regulate cell adhesion and growth. Hydrogels with cell-adhesive peptides (0.28 ± 0.03 nmol peptide cm(-2) , TTA-R-0.5; and 0.91 ± 0.12 nmol peptide cm(-2) , TTA-R-2.0) and/or micro-scaled topographical patterns (10, 25, and 80 µm grooves) are prepared using enzymatic polymerization. The adherent morphology and proliferation of C2C12 skeletal myoblasts and human aortic smooth muscle cells (hAoSM) on the hydrogels are studied. The newly developed hydrogels may be useful in investigating the roles of cell adhesion and substrate surface properties in the communication of adherent cells with the ECM.
Journal article
2012-11-01T00:00:00+00:00
12
1502 - 1513
11
Animals, Biomimetic Materials, Cell Adhesion, Cell Line, Cell Movement, Cell Proliferation, Dimethylpolysiloxanes, Extracellular Matrix, Humans, Hydrogels, Materials Testing, Mice, Microscopy, Electron, Scanning, Myoblasts, Skeletal, Myocytes, Smooth Muscle, Peptides, Surface Properties, Tissue Engineering, Tissue Scaffolds