High affinity calcium binding peptides from the gills, gut and kidneys of the freshwater eel (Anguilla anguilla).
Hearn PR., Tomlinson S., Mellersh H., Kenyon CJ., Preston CJ., Russell RG.
We report the isolation of high affinity calcium-binding peptides from homogenates of gills, gut and kidneys of the freshwater eel Anguilla anguilla. These peptides show identical gel-filtration profiles on Biogel P-6, from which a molecular weight of 3500 was estimated. The average binding constant (Kf) for calcium is 7 . 10(7)M-1, and MgCl2 up to 5 mM does not displace calcium.