Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Rabbit cortical bone powder was extracted with EDTA solutions at neutral pH and the soluble constituents fractionated by DEAE-cellulose chromatography. The acidic fractions obtained were further investigated by gel chromatography and chemical analysis. Two classes of proteoglycans were present; one containing chondroitin sulphate and the other containing material resembling keratan sulphate mixed with a smaller amount of chondroitin sulphate. Sialoglycoproteins were detected by specific chemical introduction of tritium label into the sialic acid residues of the acidic glycoprotein fraction. Following sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography, the presence of numerous distinct sialoglycoprotein components was demonstrated.

Original publication

DOI

10.1016/0003-9969(82)90164-9

Type

Journal article

Journal

Archives of oral biology

Publication Date

01/1982

Volume

27

Pages

337 - 345

Keywords

Bone and Bones, Animals, Rabbits, Edetic Acid, Macromolecular Substances, Glycosaminoglycans, Chondroitin Sulfates, Keratan Sulfate, Proteoglycans, Sialoglycoproteins, Chromatography, Electrophoresis, Polyacrylamide Gel