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The severe combined immunodeficiency (SCID) model of colitis shares many features of idiopathic inflammatory bowel disease (IBD) in humans. The SCID model is highly reproducible and easily manipulated, and as such provides a useful tool for studying mucosal immune regulation as it relates to the pathogenesis and treatment of IBD in humans. This unit describes a cell transfer system in which SCID mice are reconstituted with CD4(+) CD45RB(high) cells to induce IBD. The CD4(+) cells are isolated by immunomagnetic negative selection using monoclonal antibodies, and are then separated by fluorescent cell sorting into CD45RB(high) and CD45RB(low) cells. The former population reproducibly induces disease in recipients. A support protocol describes methods to monitor disease progression, which is characterized by weight loss, loose stools, and histologically assessed lesions in the colon.

Original publication

DOI

10.1002/0471142735.im1513s30

Type

Journal article

Journal

Current protocols in immunology

Publication Date

05/2001

Volume

Chapter 15

Pages

Unit - 15.13

Addresses

John Radcliffe Hospital, Oxford, United Kingdom.

Keywords

CD4-Positive T-Lymphocytes, Animals, Mice, Inbred BALB C, Mice, Mice, SCID, Inflammatory Bowel Diseases, Disease Models, Animal, Adoptive Transfer, Lymphocyte Depletion, Female, T-Lymphocytes, Regulatory