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This chapter provides practical information on the assay of tissue inhibitor of metalloproteinase (TIMP) activity and background information enabling meaningful interpretation of the data. Protocols are given for assessing the presence of TIMPs in biological samples by immunoblotting and by virtue of their ability to inhibit matrix metalloproteinase (MMP) hydrolysis of protein substrates (reverse zymography) and synthetic fluorogenic substrates.

Type

Chapter

Publication Date

01/2007

Volume

135

Pages

251 - 267

Keywords

Animals, Humans, Gelatin, Tissue Inhibitor of Metalloproteinases, Fluorescent Dyes, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Electrophoresis, Polyacrylamide Gel, Kinetics, Matrix Metalloproteinase Inhibitors