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GATA3 has been identified as a master regulator of T helper cells, as well as being important for early thymic progenitors and T-cell commitment. However, Gata3 expression initiates already at the hematopoietic stem cell (HSC) level, implicating a potential role also in the regulation of HSCs. Herein we used a conditional Gata3 knockout strategy in which Gata3 expression was completely deleted from the earliest stage of embryonic hematopoietic development after emergence of HSCs from hemogenic endothelium. Through a detailed analysis of HSCs at the phenotypic and functional level, we demonstrate that steady-state levels of HSCs are normal in Gata3(fl/fl)Vav-Cre(tg/+) mice. Moreover, through long-term primary and secondary transplantation experiments, we also unequivocally demonstrate that Gata3 has a redundant role in post-transplantation HSC self-renewal.

Original publication

DOI

10.1182/blood-2011-02-338046

Type

Journal article

Journal

Blood

Publication Date

08/2011

Volume

118

Pages

1291 - 1293

Addresses

Haematopoietic Stem Cell Laboratory, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom.

Keywords

Hematopoietic Stem Cells, Cells, Cultured, Animals, Mice, Inbred C57BL, Mice, Knockout, Mice, Hematopoietic Stem Cell Transplantation, Signal Transduction, Hematopoiesis, Cell Proliferation, GATA3 Transcription Factor