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Eight HLA B27-restricted influenza A virus nucleoprotein 383-391-specific cytotoxic T lymphocyte (CTL) clones were obtained from three unrelated donors following natural infection. T cell receptor (TcR) usage was studied using the "anchored" polymerase chain reaction. TcR alpha-chain usage was restricted with three predominant V alpha (V alpha 12.1, 14.1, 22) and two predominant J alpha segments. beta-chain variable-region usage was also conserved, with V beta 7 being used by five clones despite contributing less than 2% of peripheral blood lymphocyte V beta sequences of one individual studied. The TcR beta-chain junctional region was highly conserved even between CTL clones from unrelated individuals, with a negatively charged amino acid, contributed to by N-region addition, encoded at position 97 in all but two clones. This study shows that peptide-specific HLA B27-restricted CTL following influenza virus infection use very similar TcR and, when considered with previous studies, suggests a pattern of TcR conservation for major histocompatibility complex class I-restricted responses. No difference in TcR usage was detected between one healthy donor and two with HLA B27-associated arthritis.

Original publication

DOI

10.1002/eji.1830230702

Type

Journal article

Journal

Eur J Immunol

Publication Date

07/1993

Volume

23

Pages

1417 - 1421

Keywords

Amino Acid Sequence, Antigens, Viral, Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, HLA-B27 Antigen, Humans, In Vitro Techniques, Influenza A virus, Molecular Sequence Data, Nucleocapsid Proteins, Nucleoproteins, Receptors, Antigen, T-Cell, alpha-beta, Spondylitis, Ankylosing, T-Lymphocytes, Cytotoxic, Viral Core Proteins