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Oligonucleotide dendrimers were synthesized using a novel phosphoramidite synthon, tris-2,2,2-[3-(4,4'-dimethoxytrityloxy) propyloxymethyl]ethyl- N , N -diisopropylaminocyanethoxy phosphoramidite. Label, incorporated using [gamma-32P]ATP and polynucleotide kinase, was increased in proportion to the number of 5'-ends. There was a similar increase in signal when these multiply labelled oligonucleotides were used as probes to oligonucleotide arrays. A dendrimeric oligonucleotide was used successfully as a primer in the PCR. The strand bearing the dendrimer was resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply-labelled, single-stranded probe.

Original publication

DOI

10.1093/nar/25.22.4447

Type

Journal article

Journal

Nucleic acids research

Publication Date

11/1997

Volume

25

Pages

4447 - 4454

Addresses

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. misha@bioch.ox.ac.uk

Keywords

Phosphorus Radioisotopes, Biopolymers, Polynucleotide 5'-Hydroxyl-Kinase, DNA Probes, Oligonucleotides, DNA Primers, Adenosine Triphosphate, Polymerase Chain Reaction, Isotope Labeling