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The human tumor necrosis factor locus (TNF locus) is located within the major histocompatibility complex between the class III genes and HLA-B. We recently characterized and studied two closely linked highly informative dinucleotide repeats (AC/GT)n (designated TNFa) and (TC/GA)k (designated TNFb) in the upstream region of the human TNF-beta (lymphotoxin) gene. We also characterized two linked (TC/GA) and (TC/GA)-like repeats located downstream of the TNF-alpha gene, designated TNFe and TNFd, respectively. Here, we combine these four markers together with a biallelic TC/GA repeat in the first intron of the TNF-beta gene (TNFc) and a biallelic NcoI RFLP (TNFn) to type 105 cell lines from the American Society of Histocompatibility and Immunogenetics Workshop and the Center for Human Polymorphism Studies reference panels of HLA typing cell lines. These 6 polymorphic markers define 35 distinct TNF haplotypes and together with the reference panel can be used for disease association and population genetics studies.

Original publication

DOI

10.1006/geno.1993.1156

Type

Journal article

Journal

Genomics

Publication Date

04/1993

Volume

16

Pages

180 - 186

Addresses

Unit of Cytokine Molecular Biology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.

Keywords

B-Lymphocytes, Cell Line, Humans, Tumor Necrosis Factor-alpha, DNA, Genetic Markers, HLA Antigens, Histocompatibility Testing, Polymerase Chain Reaction, Base Sequence, Haplotypes, Polymorphism, Genetic, Alleles, Molecular Sequence Data