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Mechanical forces play an increasingly recognized role in modulating cell function. This report demonstrates mechanosensing by T cells, using polyacrylamide gels presenting ligands to CD3 and CD28. Naive CD4 T cells exhibited stronger activation, as measured by attachment and secretion of IL-2, with increasing substrate elastic modulus over the range of 10-200 kPa. By presenting these ligands on different surfaces, this report further demonstrates that mechanosensing is more strongly associated with CD3 rather than CD28 signaling. Finally, phospho-specific staining for Zap70 and Src family kinase proteins suggests that sensing of substrate rigidity occurs at least in part by processes downstream of T-cell receptor activation. The ability of T cells to quantitatively respond to substrate rigidly provides an intriguing new model for mechanobiology.

Original publication

DOI

10.1016/j.bpj.2011.12.011

Type

Journal article

Journal

Biophysical Journal

Publication Date

01/2012

Volume

102

Pages

L5 - L7

Addresses

Department of Biomedical Engineering, Columbia University, New York, New York, USA.

Keywords

Antigen-Presenting Cells, CD4-Positive T-Lymphocytes, Animals, Mice, Acrylic Resins, Antigens, CD28, Antigens, CD3, Antibodies, Mechanotransduction, Cellular, Mechanical Phenomena, Biomechanical Phenomena