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Cholesterol- and glycosphingolipid-enriched membrane lipid microdomains, frequently called lipid rafts, are thought to play an important role in the spatial and temporal organization of immunological synapses. Higher ordering of lipid acyl chains was suggested for these entities and imaging of membrane order in living cells during activation can therefore help to understand the mechanisms responsible for the supramolecular organization of molecules involved in the activation of T cells. Here, we employ the phase-sensitive membrane dye di-4-ANEPPDHQ together with a variety of spectrally-resolved microscopy techniques, including 2-channel ratiometric TIRF microscopy and fluorescence lifetime imaging, to characterize membrane order at the T cell immunological synapse at high spatial and temporal resolution in live cells at physiological temperature. We find that higher membrane order resides at the immunological synapse periphery where proximal signalling through the immunoreceptors and accessory proteins in microclusters has previously been shown to take place. The observed spatial patterning of membrane order in the immunological synapse depends on active receptor signalling.

Original publication

DOI

10.3109/09687688.2010.495353

Type

Journal article

Journal

Molecular membrane biology

Publication Date

08/2010

Volume

27

Pages

178 - 189

Addresses

Chemical Biology Centre, Imperial College, London, UK.

Keywords

Antigen-Presenting Cells, T-Lymphocytes, Jurkat Cells, Membrane Microdomains, Animals, Humans, Ketocholesterols, Membrane Lipids, Phosphatidylcholines, 1,2-Dipalmitoylphosphatidylcholine, Microscopy, Fluorescence, Immunological Synapses