The potent aggrecanase ADAMTS-5 is constitutively secreted by chondrocytes, but it is rapidly endocytosed in normal cartilage via the cell surface endocytic receptor LRP1. Therefore it is difficult to detect the total ADAMTS-5 activity produced. In this study, we isolated a monoclonal anti-ADAMTS-5 antibody 1B7 that blocks LRP1-mediated internalization without affecting the aggrecanolytic activity. Addition of 1B7 to cultured human chondrocytes revealed the full aggrecanolytic activity of ADAMTS-5 generated by the cells. 1B7 is a useful tool to estimate the ADAMTS-5 activity and to identify its potential roles in the tissues.
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Aggrecanase, cartilage, endocytosis, extracellular matrix, proteoglycanase, ADAMTS5 Protein, Animals, Antibodies, Monoclonal, Murine-Derived, Cattle, Chondrocytes, Humans, Low Density Lipoprotein Receptor-Related Protein-1, Mice