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Vascular endothelial cells play a pivotal role in regulating leucocyte recruitment during inflammation, and their responses can be modulated by their local environment, including cells of the tissue stroma. We have developed a model system to examine how the communication between endothelial cells and fibroblasts regulates the recruitment of leucocytes and their subsequent subendothelial fate. Here, we describe a novel co-culture filter-based flow assay and highlight the ability of synovial fibroblasts obtained from chronically inflamed tissue to promote leucocyte recruitment to otherwise 'resting' endothelial cells.

Original publication

DOI

10.1042/BST0351161

Type

Journal article

Journal

Biochem soc trans

Publication Date

11/2007

Volume

35

Pages

1161 - 1162

Keywords

Coculture Techniques, Endothelium, Vascular, Humans, Leukocytes, Stromal Cells