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We have used a 0.35-kilobase (kb) antisense RNA probe complementary to the monomorphic regions of both classical and nonclassical HLA class I sequences to detect histocompatibility-class-I-antigen-specific mRNA in human testicular tissue. The message has been clearly detected in the interstitium while less intensive staining was revealed in the peribasal compartment of the seminiferous epithelium.

Type

Journal article

Journal

Experimental and clinical immunogenetics

Publication Date

01/1993

Volume

10

Pages

202 - 207

Addresses

Institute of Human Genetics, Polish Academy of Sciences, Poznan.

Keywords

Testis, Humans, Antisense Elements (Genetics), RNA, Antisense, RNA, Messenger, Histocompatibility Antigens Class I, In Situ Hybridization, Gene Expression, Tissue Distribution, Male