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We have analyzed the brain distribution of the rat cAMP-specific phosphodiesterases (rPDEIV) which are closely related to the defective gene products of the drosophila melanogaster learning and memory mutant dunce. PCR analysis of rat brain cDNA was performed on the four known dunce-like cAMP PDE rat isogenes (rPDE-IV-A, -B, -C, -D). High expression of three of these isogenes (rPDEIV-A, -B, -D) highlighted their involvement in regulation of cAMP in the brain. Specific probes for all four isogenes were then used for in situ hybridization of rat brain sections. Distinct but overlapping expression patterns were observed for rPDEIV-A, rPDEIV-B, and rPDEIV-D. Abundant expression of these subtypes was observed in the olfactory system, the hippocampus and the cerebellum, while no specific signals could be detected in most areas of the brain for the subtype rPDEIV-C.

Type

Journal article

Journal

Journal of neuroscience research

Publication Date

06/1995

Volume

41

Pages

169 - 178

Addresses

Preclinical Research, Sandoz Pharma, Ltd., Basel, Switzerland.

Keywords

Hippocampus, Olfactory Bulb, Animals, Rats, Rats, Wistar, Cyclic AMP, In Situ Hybridization, Polymerase Chain Reaction, Sequence Analysis, DNA, Memory, Brain Chemistry, Base Sequence, Long-Term Potentiation, Molecular Sequence Data, Male, 3',5'-Cyclic-AMP Phosphodiesterases