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The ability of fibroblastic cells to respond to parathyroid hormone (PTH) by an increase in adenylate cyclase activity is accepted as a characteristic of the osteogenic phenotype. Whether marrow fibroblastic cells, which have osteogenic potential when assayed in vivo, demonstrate this hormonal response when cultured in vitro has been investigated. Our study has shown a level of stimulation of adenylate cyclase activity by PTH in cultured rabbit marrow fibroblasts comparable with other osteogenic cells in vitro. The effect is seen in fibroblasts grown either from multiple colonies or from single colonies. Only a proportion of colonies had osteogenic potential in vivo assay and our results show a similar finding for the PTH response in vitro. To what degree the two parameters are expressed by the same colony has not yet been established.

Original publication

DOI

10.1016/0169-6009(89)90060-3

Type

Journal article

Journal

Bone and mineral

Publication Date

08/1989

Volume

7

Pages

23 - 30

Addresses

Department of Biochemistry, University of Auckland, New Zealand.

Keywords

Bone Marrow Cells, Cells, Cultured, Cell Line, Fibroblasts, Bone Marrow, Animals, Rabbits, Osteosarcoma, Tritium, Isoproterenol, Parathyroid Hormone, Adenylate Cyclase, Cyclic AMP, Enzyme Activation, Kinetics, Male