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A monoclonal antibody of immunoglobulin class G1 has been produced which reacts with a high molecular weight antigen apparently present exclusively in osteogenic tissues. Immunohistochemical studies have shown that the antigen is present throughout the mineralized matrix and in osteoid. None of the other tissues examined namely liver, intestine, kidney, spleen, thymus, heart, lung, skin, cartilage and skeletal muscle showed evidence of specific antibody binding. Immunohistochemical staining was also demonstrated in tissues developing from rabbit marrow cultured in vitro and in diffusion chambers in vivo. Temporal studies of antigen expression in the chambers indicated that the antigen occurs at sites of bone formation after the appearance of alkaline phosphatase but before the formation of a mineralized matrix. The results of these studies suggest that the monoclonal antibody recognises a product of differentiated osteoblasts. This antibody may therefore prove useful in studies of osteogenic differentiation.

Original publication

DOI

10.3109/03008208909005628

Type

Journal article

Journal

Connective tissue research

Publication Date

01/1989

Volume

23

Pages

289 - 297

Addresses

Nuffield Department of Orthopaedic Surgery, Nuffield Orthopaedic Centre, Oxford, England.

Keywords

Bone and Bones, Osteoblasts, Animals, Rabbits, Antibodies, Monoclonal, Immunohistochemistry, Osteogenesis