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A variety of analyses, including fluorescence in situ hybridization (FISH), quantitative PCR (qPCR) and array CGH (aCGH), have been performed on a series of chordomas from 181 patients. Twelve of 181 (7%) tumours displayed amplification of the T locus and an additional two cases showed focal amplification; 70/181 (39%) tumours were polysomic for chromosome 6, and 8/181 (4.5%) primary tumours showed a minor allelic gain of T as assessed by FISH. No germline alteration of the T locus was identified in non-neoplastic tissue from 40 patients. Copy number gain of T was seen in a similar percentage of sacrococcygeal, mobile spine and base of skull tumours. Knockdown of T in the cell line, U-CH1, which showed polysomy of chromosome 6 involving 6q27, resulted in a marked decrease in cell proliferation and morphological features consistent with a senescence-like phenotype. The U-CH1 cell line was validated as representing chordoma by the generation of xenografts, which showed typical chordoma morphology and immunohistochemistry in the NOD/SCID/interleukin 2 receptor [IL2r]gammanull mouse model. In conclusion, chromosomal aberrations resulting in gain of the T locus are common in sporadic chordomas and expression of this gene is critical for proliferation of chordoma cells in vitro.

Original publication

DOI

10.1002/path.2816

Type

Journal article

Journal

The Journal of pathology

Publication Date

02/2011

Volume

223

Pages

327 - 335

Addresses

UCL Cancer Institute, 72 Huntley Street, London WC1 6BT, UK.

Keywords

Tumor Cells, Cultured, Animals, Humans, Mice, Mice, SCID, Chordoma, Chromosome Aberrations, Genetic Predisposition to Disease, T-Box Domain Proteins, Fetal Proteins, Neoplasm Proteins, DNA, Neoplasm, Transplantation, Heterologous, In Situ Hybridization, Fluorescence, Polymerase Chain Reaction, Neoplasm Transplantation, Cell Proliferation, Gene Knockdown Techniques, DNA Copy Number Variations