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Homodimerization is an essential step for membrane type 1 matrix metalloproteinase (MT1-MMP) to activate proMMP-2 and to degrade collagen on the cell surface. To uncover the molecular basis of the hemopexin (Hpx) domain-driven dimerization of MT1-MMP, a crystal structure of the Hpx domain was solved at 1.7 Å resolution. Two interactions were identified as potential biological dimer interfaces in the crystal structure, and mutagenesis studies revealed that the biological dimer possesses a symmetrical interaction where blades II and III of molecule A interact with blades III and II of molecule B. The mutations of amino acids involved in the interaction weakened the dimer interaction of Hpx domains in solution, and incorporation of these mutations into the full-length enzyme significantly inhibited dimer-dependent functions on the cell surface, including proMMP-2 activation, collagen degradation, and invasion into the three-dimensional collagen matrix, whereas dimer-independent functions, including gelatin film degradation and two-dimensional cell migration, were not affected. These results shed light on the structural basis of MT1-MMP dimerization that is crucial to promote cellular invasion.

Original publication

DOI

10.1074/jbc.m110.178434

Type

Journal article

Journal

The Journal of biological chemistry

Publication Date

03/2011

Volume

286

Pages

7587 - 7600

Addresses

Arbeitsgruppe Proteinaseforschung, Max-Planck-Institut fuer Biochemie, Am Klopferspitz 18, D-82152 Martinsried, Germany.

Keywords

COS Cells, Hela Cells, Extracellular Matrix, Animals, Cercopithecus aethiops, Humans, Hemopexin, Membrane Proteins, Crystallography, Mutagenesis, Enzyme Activation, Protein Structure, Tertiary, Structure-Activity Relationship, Dimerization, Solubility, Matrix Metalloproteinase 14