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Glucocorticoids (GCs), which are used in the treatment of immune-mediated inflammatory diseases, inhibit the expression of many inflammatory mediators. They can also induce the expression of dual specificity phosphatase 1 (DUSP1; otherwise known as mitogen-activated protein kinase [MAPK] phosphatase 1), which dephosphorylates and inactivates MAPKs. We investigated the role of DUSP1 in the antiinflammatory action of the GC dexamethasone (Dex). Dex-mediated inhibition of c-Jun N-terminal kinase and p38 MAPK was abrogated in DUSP1-/- mouse macrophages. Dex-mediated suppression of several proinflammatory genes (including tumor necrosis factor, cyclooxygenase 2, and interleukin 1alpha and 1beta) was impaired in DUSP1-/- mouse macrophages, whereas other proinflammatory genes were inhibited by Dex in a DUSP1-independent manner. In vivo antiinflammatory effects of Dex on zymosan-induced inflammation were impaired in DUSP1-/- mice. Therefore, the expression of DUSP1 is required for the inhibition of proinflammatory signaling pathways by Dex in mouse macrophages. Furthermore, DUSP1 contributes to the antiinflammatory effects of Dex in vitro and in vivo.

Original publication

DOI

10.1084/jem.20060336

Type

Journal article

Journal

The Journal of experimental medicine

Publication Date

08/2006

Volume

203

Pages

1883 - 1889

Addresses

Kennedy Institute of Rheumatology Division, Imperial College London, Hammersmith, London W6 8LH, United Kingdom.

Keywords

Macrophages, Bone Marrow, Animals, Mice, Inflammation, Dexamethasone, JNK Mitogen-Activated Protein Kinases, p38 Mitogen-Activated Protein Kinases, Cell Cycle Proteins, Immediate-Early Proteins, RNA, Messenger, Anti-Inflammatory Agents, Glucocorticoids, Signal Transduction, Gene Expression Regulation, Enzyme Induction, Dose-Response Relationship, Drug, Phosphoprotein Phosphatases, Protein Tyrosine Phosphatases, Dual Specificity Phosphatase 1, Protein Phosphatase 1