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Within 6 h, shear stress upregulated intercellular adhesion molecule-1 (ICAM-1) (two- to four-fold, P<0.001) and induced matrix metalloproteinase-2 (MMP-2) in cultured human saphenous vein endothelial cells. By 8 h endothelial ICAM-1 levels returned to baseline, with concomitant increase in soluble ICAM-1 (sICAM-1) (P<0.001) and MMP-9 had been induced. Inclusion of a hydroxamate metalloproteinase inhibitor partially reversed the effects on ICAM-1 and sICAM-1 at 8 h, whereas TIMP-1, -2 or -3 had no effect. MMP-9, but not MMP-2, co-immunoprecipitated with ICAM-1. sICAM-1 was processed distal to Arg441, indicating that MMP-9, docking to ICAM-1, contributes to sICAM-1 shedding and attenuation of the shear stress-induced upregulation of ICAM-1.

Original publication

DOI

10.1016/s0014-5793(04)00337-0

Type

Journal article

Journal

FEBS letters

Publication Date

04/2004

Volume

564

Pages

161 - 165

Addresses

Department of Vascular Surgery (Charing Cross site), Imperial College London, St Dunstan's Road, London W6 8RP, UK.

Keywords

Endothelium, Vascular, Saphenous Vein, Humans, Intercellular Adhesion Molecule-1, Enzyme Induction, Protein Processing, Post-Translational, Up-Regulation, Protein Binding, Solubility, Stress, Mechanical, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9