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This chapter provides information on imaging tools that can be employed to visualise and study lymphoid organ development. We focus on the use of genetically modified mouse models that take advantage of fluorescent protein expression in discrete cell populations, thus allowing live cell imaging during lymphoid organogenesis. We discuss approaches that allow characterisation of the cell types involved in the formation of lymphoid organs, including (i) functional assays in explant organ cultures and (ii) high-resolution whole-mount immunostaining methods, which are useful for the characterisation of specific cell populations in the context of the whole developing organ.

Original publication

DOI

10.1007/978-1-60761-461-6_11

Type

Journal article

Journal

Methods mol biol

Publication Date

2010

Volume

616

Pages

161 - 179

Keywords

Animals, Cell Lineage, Cell Survival, Dissection, Embryo, Mammalian, Humans, Imaging, Three-Dimensional, Luminescent Proteins, Lymphatic System, Mice, Models, Animal, Organogenesis, Staining and Labeling