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Platelet rich plasma (PRP) is the autologous plasma fraction with a platelet-rich cellular component which is enriched with a number of growth factors. Due to its availability and low cost, PRP has become an increasingly popular clinical tool as an alternative source of growth factors for various applications, for example, tendon regeneration but with limited success in clinical trials. The main objective of the current study was to determine whether activated PRP [i.e., platelet rich plasma-clot release (PRCR)] could be used to induce the proliferation and collagen synthesis in human tenocyte in vitro. The advantage of using PRCR is that the platelet-derived bioactive factors are more concentrated and could initiate a more rapid and accelerated healing response than PRP. Our results demonstrated that 10% PRCR treatment accelerated the extent of cell proliferation and collagen production by human tenocytes in vitro. The expression of specific tenocyte markers were similar to conventional fetal bovine serum (FBS)-treated tenocytes implanted in mice within 14 days of implantation in diffusion chambers. Moreover, relatively more collagen fibrils were evident in PRCR-treated tenocytes in vivo as compared to 10% FBS-treated cells. Overall, our feasibility study has indicated that PRCR can induce human tenocyte proliferation and collagen synthesis which could be implemented for future tendon regeneration in reconstructive surgeries.

Original publication

DOI

10.1002/jor.22016

Type

Journal article

Journal

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Publication Date

06/2012

Volume

30

Pages

982 - 990

Addresses

Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford OX3 7LD, UK. goodthing1209@gmail.com

Keywords

Tendons, Cells, Cultured, Fetal Blood, Animals, Cattle, Humans, Collagen Type I, Culture Media, Clot Retraction, Diffusion Chambers, Culture, Wound Healing, Cell Differentiation, Cell Proliferation, Gene Expression, Adult, Middle Aged, Female, Male, Basic Helix-Loop-Helix Transcription Factors, Platelet-Rich Plasma, Decorin