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The osteoclastogenic factor of osteoblastic origin has recently been elucidated as a novel Tumor Necrosis Factor (TNF)-ligand family member, termed osteoclast differentiation factor (ODF). Using a semiquantitative RT-PCR approach, we sought to determine the mRNA expression of ODF and its decoy receptor, osteoprotegerin (OPG), in a selection of osteoblastic cell lines and in response to three factors representative of different signal transduction pathways, vitamin D receptor, protein kinase A or gp130. Each osteotropic agent, either 1,25-(OH)2D3, PTH or IL-11, promoted an increase in the ratio of ODF:OPG, with maximal stimulation occurring at 24 h, 4 h, and 8 h, respectively, and furthermore each was shown to act in a dose-dependent manner. This report establishes that osteoblastic cell lines incapable of supporting osteoclast formation have markedly reduced ODF expression and also illustrates the importance of the relative abundance of ODF compared with the levels of OPG for the induction of osteoclastogenesis.

Original publication

DOI

10.1210/en.139.11.4743

Type

Journal article

Journal

Endocrinology

Publication Date

11/1998

Volume

139

Pages

4743 - 4746

Addresses

St. Vincent's Institute of Medical Research and The University of Melbourne, Department of Medicine, St. Vincent's Hospital, Fitzroy, Victoria, Australia.

Keywords

Cells, Cultured, Osteoclasts, Osteoblasts, Stromal Cells, Animals, Mice, Inbred C57BL, Animals, Newborn, Mice, Calcitriol, Parathyroid Hormone, Cyclic AMP-Dependent Protein Kinases, Glycoproteins, Carrier Proteins, Membrane Glycoproteins, Receptors, Tumor Necrosis Factor, Receptors, Cytoplasmic and Nuclear, Receptors, Calcitriol, Interleukin-11, Cytokines, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, RANK Ligand, Receptor Activator of Nuclear Factor-kappa B, Osteoprotegerin