Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Mouse dendritic cells (DCs) can rapidly extend their Class II MHC-positive late endosomal compartments into tubular structures, induced by Toll-like receptor (TLR) triggering. Within antigen-presenting DCs, tubular endosomes polarize toward antigen-specific CD4(+) T cells, which are considered beneficial for their activation. Here we describe that also in human DCs, TLR triggering induces tubular late endosomes, labeled by fluorescent LDL. TLR triggering was insufficient for induced tubulation of transferrin-positive endosomal recycling compartments (ERCs) in human monocyte-derived DCs. We studied endosomal remodeling in human DCs in co-cultures of DCs with CD8(+) T cells. Tubulation of ERCs within human DCs requires antigen-specific CD8(+) T cell interaction. Tubular remodeling of endosomes occurs within 30 min of T cell contact and involves ligation of HLA-A2 and ICAM-1 by T cell-expressed T cell receptor and LFA-1, respectively. Disintegration of microtubules or inhibition of endosomal recycling abolished tubular ERCs, which coincided with reduced antigen-dependent CD8(+) T cell activation. Based on these data, we propose that remodeling of transferrin-positive ERCs in human DCs involves both innate and T cell-derived signals.

Original publication




Journal article


J biol chem

Publication Date





520 - 528


Cell-Cell Interaction, Cross-presentation, Dendritic Cells, Endosomes, Intracellular Trafficking, Live Cell Confocal Microscopy, Recycling, T Cell Receptor, Toll-like Receptor (TLR), Tubulation, Animals, Antigen Presentation, CD8-Positive T-Lymphocytes, Cells, Cultured, Coculture Techniques, Dendritic Cells, Endosomes, Female, HLA-A2 Antigen, Humans, Immunity, Cellular, Immunity, Innate, Intercellular Adhesion Molecule-1, Lymphocyte Function-Associated Antigen-1, Male, Mice, Monocytes, Signal Transduction, Toll-Like Receptors