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Lymphoid cells of CBA mice were triggered to act as specific helper cells by incubation with protein antigen (usually keyhole limpet haemocyanin (KLH)) in Marbrook cultures in vitro. After optimum priming, these helper cells, at optimal numbers, stimulated B cells (from unprimed spleens) to respond to trinitrophenyl-KLH in vitro. The in vitro-induced helper cells were carrier-specific. B cell depletion before helper cell induction increased the efficiency of helper cell induction and thus provided further proof of the T-cell nature of in vitro helper cells. Heterogeneity within T helper cell precursors is suggested on the basis of differences in antigen dose requirements of precursor cells in cortisone-resistant thymocytes, spleen, or lymph nodes.

Original publication

DOI

10.1111/j.1365-3083.1975.tb02608.x

Type

Journal article

Journal

Scandinavian journal of immunology

Publication Date

01/1975

Volume

4

Pages

121 - 128

Keywords

Lymph Nodes, Spleen, B-Lymphocytes, T-Lymphocytes, Cells, Cultured, Animals, Mice, Inbred CBA, Chickens, Cattle, Mice, Hemocyanin, gamma-Globulins, Immune Sera, Cell Count, Dose-Response Relationship, Drug