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Cell-cell adhesion is essential for many immunological functions. The LFA-1 molecule, a member of a superfamily of adhesion molecules, participates in adhesion which is critical to the function of each of the three major subsets of leukocytes: lymphocytes, monocytes and granulocytes. Putative LFA-1 ligands have been identified functionally in different laboratories using three different monoclonal antibodies that inhibit LFA-1-mediated leukocyte adhesion in particular model systems; however, there may be more than one LFA-1 ligand. We have directly compared the three relevant monoclonal antibodies, and show that each binds to the same molecule, intercellular-adhesion molecule-1 (ICAM-1). Most important, B, T and myeloid cells adhere specifically to purified ICAM-1-coated surfaces; such adhesion has distinctive requirements for Mg2+ and Ca2+. This constitutes biochemical evidence that ICAM-1 functions as a ligand for LFA-1-dependent adhesion by a variety of leukocytes.

Original publication

DOI

10.1038/331086a0

Type

Journal article

Journal

Nature

Publication Date

07/01/1988

Volume

331

Pages

86 - 88

Keywords

Antibodies, Monoclonal, Antigens, Surface, B-Lymphocytes, Calcium, Cell Adhesion, Hematopoietic Stem Cells, Lymphocyte Function-Associated Antigen-1, Magnesium, T-Lymphocytes, Cytotoxic, Tumor Cells, Cultured